شناسایی پلی مورفیسم G276T از ژن آدیپونکتین با استفاده از تکنیک PCR-RFLP در افراد مبتلا به سرطان پستان

نویسنده

استادیار گروه زیست شناسی،دانشگاه آزاد اسلامی واحد رودهن

چکیده

مقدمه: هورمون آدیپونکتین در تعدیل فرایندهای متابولیکی همچون تنظیم گلوکز و کاتابولیسم اسیدهای چرب نقش دارد. چند پلی‌مورفیسم تک نوکلئوتیدی (SNP) در منطقه رونویسی از ژن و توالی‌های اطراف آن در جمعیت‌های مختلف شناسایی شده که با شیوع بیماری های مختلفی مرتبط هستند. هدف از این مطالعه تعیین فراوانی پلی مورفیسم G276T در افراد مبتلا به سرطان پستان و مقایسه آن با افراد سالم بود.
روش‌ کار: 128 فرد مبتلا به سرطان پستان و 128 فرد سالم پس از تایید پزشک متخصص انتخاب شدند. پس از خون گیری و استخراج DNA ، تمامی نمونه‌ها با پرایمرهای اختصاصی تکثیر شدند؛ سپس بخشی از آن ها با آنزیم Mva1269I برش داده شدند تا ژنوتایپ افراد تعیین شود. نتایج به دست آمده با نسخه 23 نرم افزار IBM SPSS  و به کمک آزمون های آماری کای مربع و رگرسیون لوجستیک مورد بررسی قرار گرفت.
یافته­ ها: دو گروه تفاوت معناداری برای میانگین سنی و شاخص توده بدنی نداشتند. ژنوتیپ GT در دو گروه تفاوت معناداری داشت و   شانس ابتلا به سرطان پستان را به میزان 1.86 برابر در حاملین­ افزایش داد. هیچ کدام از ژنوتایپ ها با تغییرات هیستوپاتولوژی ارتباطی نداشتند.
نتیجه‌گیری: الل موتانت T در موقعیت G276T آدیپونکتین، اثر کاهشی بر سرطان پستان نشان داد که این نتیجه نیاز به بررسی در جمعیت های مختلف با تعداد نمونه بیشتر برای تایید شدن دارد.
 

کلیدواژه‌ها

عنوان مقاله [English]

Detection of G276T Polymorphism from Adiponectin Gene Using PCR-RFLP Technique in Patients with Breast Cancer

نویسنده [English]

  • Zahra Tahmasebi Fard

Assistant professor of biology group. Islamic Azad of university

چکیده [English]

Introduction: Adiponectin hormone is involved in modulating metabolic processes such as glucose regulation and
fatty acid catabolism. Several single nucleotide polymorphisms (SNPs) in the transcription region of the gene and its sequences have been identified in different populations that are associated with the prevalence of various diseases. The aim of this study was to determine frequency of G276T polymorphism in breast cancer patients and its comparing in subjects without breast cancer.
Materials and Methods: 128 individuals with breast cancer and 128 healthy people were selected after the approval of a specialist physician. After blood collection and DNA extraction, all specimens were amplified with specific primers, then part of them was cut with Mva1269I enzyme and the genotypes of individuals were determined. The results were analyzed using IBM SPSS version 23 software by chi-square and logistic regression tests.
Results: The two groups did not have a significant difference for mean age and body mass index. GT genotype was significantly different between the two groups and increased the risk of breast cancer by 1.86 folds in their carriers. None of the genotypes had any relationship with histopathological changes.
Conclusion: The T mutant allele at G276T position of adiponectin had a decreasing effect on breast cancer, indicating that this result needs to be investigated in different populations with a larger sample size for confirmation.

کلیدواژه‌ها [English]

  • Breast Cancer
  • Polymorphism
  • Adiponectin
  • RFLP
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