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:: Volume 3, Issue 3 (Fall 2006) ::
jmj 2006, 3(3): 1-7 Back to browse issues page
Effect of Vero cell Co – culture on Quality of in vitro produced bovine blastocysts after vitrification
H Karimi Jashni *, Mohammad Hossein Nasr esfhani, Hossein Emani, Mohammad Mardani
Abstract:   (3875 Views)

Introduction : Post freze – thaw embryo quality can be assessed by various methods , the most common being in vetro survival after a period of culture . several culture system have been used for this purpose and most of them include somatic cell such as oviduct , granulosa and  Buffalo rat liver cells as Co- culture support . The aim of this study was to assess the Vero cell Co- culture system on quality of in vitro produced bovine blastocysts after vitrification.

Materials & Methods :after in vitro maturation and fertilization of Immature bovine oocytes . presumptive zygotes were cultured in SoFAWT IMAGEand SoFAWT IMAGE medium for 7 days (39 c , 5% CoAWT IMAGE) . Then 100 blastorcysts were selected and vitrified  ( 40% ethylene glycol , 18% ficol , 0.3 mol sacurose).After tawing blastocysts were divided into two groups . One group was cultured in SOFAWT IMAGE : and the other group was cultured in SoFAWT IMAGE + Vero cell medium for 48 h. re-expanded blastocysts were selected, inner cell mass(ICM) from them were recovered by immunosurgery and stained cell with Hoechst .

Results : The mean number of ICM in SoFAWT IMAGE and SoFAWT IMAGE + Vero were 12 and 15 respectively . The percentage re-expanded blastocysts in SoFAWT IMAGEand SoFAWT IMAGE+ Vero were 42%,54% respectivety . there were significant difference between the number of ICM and percentage re–expanded blastocysts in the two groups.

Conclusion : Vero cell Co – culture system can improve viability of in vitro produced bovine blastosysts after vitrification . 

Keywords: Bovine blastocyst – Vero cell - Inner cell mas - Vitrification
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Type of Study: Research | Subject: ect
Received: 2016/07/16 | Revised: 2018/02/28 | Accepted: 2016/07/16
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Karimi Jashni H, Nasr esfhani M H, Emani H, Mardani M. Effect of Vero cell Co – culture on Quality of in vitro produced bovine blastocysts after vitrification. jmj 2006; 3 (3) :1-7
URL: http://jmj.jums.ac.ir/article-1-841-en.html

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Volume 3, Issue 3 (Fall 2006) Back to browse issues page
فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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