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:: Volume 2, Issue 2 (spring 2005) ::
jmj 2005, 2(2): 6-11 Back to browse issues page
Value of Urease Test in the detection of Helicobacter pylori infections
Reza Gottaslou *, Bahram Kazemi, Faransis Megraud, Hossein Eliasi, Ahad Zargarizadeh, Mohammad Rakhshan, Hossein Goodarzi
Abstract:   (20347 Views)

Introduction: H. pylori is microaerophilic, garm negative, spiral organism with 4-6 flagella. The bacterium is an etiologic agent for chronic gastritis and plays a major role in effecting peptic ulcer, gastric cancer, lymphoma. H. pylori infection has a high seroprevalence in Iran (> 90%). Thus diagnosis and  treatment of H.pylori infection is very important.

Material and Methods : The presence of H. pylori infection was determined in 100 patients undergoing upper gastrointestinal endoscopy over 9 month period. The presence of H. pyolri was detcted in gastric mucosal biopsy specimens by Biopsy Urease Test, PCR and histopathology. A media was used for urease test, Normal Broth Urea. The biopsy is placed in a broth containing urea and a PH indicator. The goldstandard is histopathology examination. We designed a PCR for amplifying the H. pylori gene encoding ure C (294 bp).

Results : The results of urease test were 66.6% sensitivity and 69.7% specefity. The results of PCR assay were 98.6% sensitivity and 93% specifity. There was a significant difference between the specifity and sensitivity of urease test and PCR.

Conclusion : The result of This study showed  that urease test was not a sensitive and specific method for accurate detection of  the infection. It a level of the sensitivity is too low to be used as the sole method of detection in ordinary practic. In this situation other/ tests should be used.

Keywords: Helicobacter pylori, Urease test, Polymerase chain reaction (PCR)
Full-Text [PDF 130 kb]   (1170 Downloads)    
Type of Study: Research | Subject: ect
Received: 2016/07/11 | Revised: 2018/03/4 | Accepted: 2016/07/11
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Gottaslou R, Kazemi B, Megraud F, Eliasi H, Zargarizadeh A, Rakhshan M et al . Value of Urease Test in the detection of Helicobacter pylori infections. jmj. 2005; 2 (2) :6-11
URL: http://jmj.jums.ac.ir/article-1-827-en.html

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Volume 2, Issue 2 (spring 2005) Back to browse issues page
فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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