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Effect of Cyclophosphamide on miRNA-15a expression in EBV infected transformed B cell
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Abdolreza Sotoodeh Jahromi  , Mohammad Kargar , Maliheh Moradzadeh , Farshid Kafilzadeh , Marzieh Jamalidoust |
| Golestan University of Medical Sciences, Gorgan, Iran |
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Abstract: (97 Views) |
Background: Despite advances in diagnosis and therapy of cancer, it is still the main cause of death worldwide. Cyclophosphamide is one of the major drugs for lymphoma treatment. However, the anti-lymphoma effects of cyclophosphamide on micro-RNA expression are still unclear, especially in B cell lymphomas. The aim of the present study was to evaluate the effect of cyclophosphamide on miRNA-15a genes expression in Epstein‐Barr‐Virus (EBV)-infected transformed B cell.
Material and Methods: In this study, the effects of cyclophosphamide on CO 88BV59-1 LCL – an EBV infected transformed B cell was evaluated. The cells were treated with cyclophosphamide (0.05-50 µM), for 3 days. The cell viability and cytotoxicity were evaluated using MTT, and flow cytometry techniques, respectively. Also, miRNA-15a genes expression were determined by real-time PCR.
Results: Cyclophosphamide significantly inhibited proliferation and induced cell death in the cell-line in a dose and time-dependent manner (P<0.05). There were no significant changes in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05).
Conclusions: The results showed that cytotoxic effects of cyclophosphamide on CO 88BV59-1 LCL is not related to miRNA-15a3p and miRNA-15a5p expressions. |
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| Keywords: Cyclophosphamide, Micro-RNA, B cell, Epstein Barr virus |
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Type of Study: Research |
Subject:
daro sazi
Received: 2021/01/16 | Accepted: 2021/03/17 | Published: 2021/03/17
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