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Effect of Crocin on cell apoptosis and miRNA-15a expression in EBV infected transformed B cell-CO 88BV59-1 LCL
Abdolreza Sotoodeh Jahromi , Maliheh Moradzadeh , Mohammad Kargar , Farshid Kafilzadeh , Marzieh Jamalidoust
Jahrom branch, Islamic Azad University
Abstract:   (60 Views)
Background: Despite advances in diagnosis and therapy, cancer is still the main cause of death worldwide. Crocin, the major carotenoid in saffron, exhibits potent anti-tumor effects in breast, pancreas and gastric tumors. However, the anti-lymphoma effects of crocin are still unclear, especially in B cell lymphomas. The aim of the present study was to evaluate the cytotoxic and apoptotic effects of crocin on EBV-infected B cell lymphoma.
Material and Methods: In this study, the effects of crocin on CO 88BV59-1 LCL – an Epstein‐Barr‐Virus (EBV) Infected Transformed B cell was examined. The cells were treated with crocin (0.2-200 µM), for 3 days. The cell viability, and apoptosis were evaluated using MTT, and flow cytometry techniques respectively. Also, miRNA-15a genes expression were determined by real-time PCR.
Results: Crocin significantly inhibited proliferation and induced apoptosis in EBV infected cell-line in a dose and time-dependent manner (P<0.05). There was no significant change in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05).
Conclusions: The results suggested that crocin can be considered for preclinical and also clinical testing in EBV-associated B cell lymphoma treatment. The results showed that due to the effect of crocin in reducing the survival and increasing apoptosis of lymphoma cells and also due to the lack of change in the expression of miRNA-15a, it can be considered as a therapeutic candidate in the treatment of EBV-associated B cell lymphoma.
Keywords: Apoptosis, B cell lymphoma, Crocin
     
Type of Study: Research | Subject: ect
Received: 2020/11/8 | Accepted: 2020/11/15
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Research code: IR.JUMS.REC.1399.026
Ethics code: IR.JUMS.REC.1399.026


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فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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